fluorescence intensity in zen lite software

Curr Opin Microbiol 14:188–193īottomley MJ, Muraglia E, Bazzo R, Carfì A (2007) Molecular insights into quorum sensing in the human pathogen Pseudomonas aeruginosa from the structure of the virulence regulator LasR bound to its autoinducer. This is probably due to selectivity for cognate AHL molecules of appropriate concentrations.Īhmer BMM, Soares JA (2011) Detection of acyl-homoserine lactones by Escherichia and Salmonella. aeruginosa did not interact with any of the three conjugates, whereas C.

coli showed emission with all the AHL conjugates and P. In the concentration employed, selectivity to a limited extent is observed between the three Gram-negative bacteria tested. AuNCs are then functionalized with the signal molecules, Acyl Homoserine Lactones (AHL) of varying carbon chain length, C-6, C-8, and C-12 without 3rd C modification, to sense different strains of gram-negative bacteria i.e., Escherichia coli, Cronobacter sakazakii and Pseudomonas aeruginosa. Here, this property is exploited for understanding the quorum sensing phenomenon in bacteria which is regulated by signal molecules which are specific to various species.

Ultra-small (size < 2 nm) gold nanoclusters (AuNCs) are used as fluorescent probes which have excellent applications in bioimaging and sensing due to their emission in visible and NIR spectral region.

Fluorescence intensity in zen lite software